<i>In vitro</i> assessment of protein digestibility and mineral solubility of black soldier fly larvae meals for monogastric animals

Animal farming is a fast-growing sector which demands large supply of feed materials and the search for novel ingredients with smaller impact on global environment has been intensified. However, before inclusion these in animal feed, preliminary assessment quality performance needed. This evaluation usually done by vivo feeding trials, but vitro digestion methods have successfully used gathering information regarding nutritional value different components. Therefore, this work, an method was developed using black soldier fly (BSF) larvae meals. The included seven experimental variables, namely: pH stomach, intestine, reaction time enzyme concentration intestine temperature. variables were screened means fractional factorial design (2 7-2 ) that allowed to select temperature (at 7 U 37 °C, respectively) as most influential factors amino acid mineral solubility BSF meal. Performing digestibility at °C stomach 2 9, 1 hour 4 hours resulted highest overall nutrient solubility. optimised methodology assess stability three batches meals delivered same producer. able reveal differences between